Rationally engineered Cas9 nucleases with improved specificity
نویسندگان
چکیده
منابع مشابه
Rationally engineered Cas9 nucleases with improved specificity.
The RNA-guided endonuclease Cas9 is a versatile genome-editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites that are not fully complementary to the guide, which poses a major challenge for genome editing. ...
متن کاملRationally engineered Cas 9 nucleases with improved specificity
1. A. M. Zaki, S. van Boheemen, T. M. Bestebroer, A. D. Osterhaus, R. A. Fouchier, N. Engl. J. Med. 367, 1814–1820 (2012). 2. A. Bermingham et al., Euro Surveill. 17, 20290 (2012). 3. World Health Organization, Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (2015); www.who.int/emergencies/ mers-cov/en/. 4. C. B. E. M. Reusken et al., Lancet Infect. Dis. 13, 859–866 (2013). 5. A. N. Ala...
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tp://dx.doi.org/10.1016/j.gendis.2 52-3042/Copyright a 2014, Chongq Bacteria and archaea have been known for decades having evolvedadaptive immunedefenses calledclustered regularly interspaced short palindromic repeats (CRISPR)/CRISPRassociated (Cas) systems to degrade foreign nucleic acids. Recently, these RNA-guided Cas9 nucleases derived from CRISPR/Cas systems have shown promise in transfor...
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ژورنال
عنوان ژورنال: Science
سال: 2015
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.aad5227